reversetranscriptasepcr

With two-step RT-PCR, the RNA is first reverse transcribed into cDNA using oligo-dT primers, random oligomers, or gene-specific primers. An aliquot of the ...

Reverse transcription PCR, or RT-PCR, allows the use of RNA as a template. An additional step allows the detection and amplification of RNA. The RNA is reverse ...

由 SL Emery 著作 · 2004 · 被引用 393 次 — A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated ...

2020年6月15日 — The final acronym 'RT-qPCR' is used for reverse transcription quantitative real-time PCR. This is a technique which combines RT-PCR with qPCR to ...

由 G Bleve 著作 · 2003 · 被引用 228 次 — Reverse transcriptase PCR (RT-PCR) and real-time RT-PCR assays have been used to detect and quantify actin mRNA from yeasts and molds.

Reverse transcriptase (RT) is the enzyme that makes DNA from RNA. Some reverse transcriptases have RNase activity to degrade the RNA strand in the RNA-DNA ...